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  4. Phospho-CREB1 (S133) Recombinant Monoclonal Antibody

Phospho-CREB1 (S133) Recombinant Monoclonal Antibody

  • 中文名稱：
    磷酸化-CREB1 (S133)重組抗體
  • 貨號：
    CSB-RA005947A133phHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in Hela whole cell lysate,293 whole cell lysate
      All lanes Phospho-CREB1 antibody at 1.65μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 46 KDa
      Observed band size: 46 KDa
    • IHC image of CSB-RA005947A133phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA005947A133phHU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-RA005947A133phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    The vectors expressing anti-CREB1 antibody were constructed as follows: immunizing an animal with a synthesized peptide derived from human Phospho-CREB1 (S133), isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening CREB1 antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant CREB1 antibody. Recombinant CREB1 antibody in the culture medium was purified using affinity-chromatography. It can react with CREB1 protein from Human and is used in the ELISA, WB, IHC, IF.

    In adult mammalian retina, p-CREB1 is normally limited to the ganglion cell and inner nuclear layers. It appears that as in other parts of the nervous system, stressful stimuli can induce phosphorylation of CREB1 in retinal neurons. CREB1 not only controls the expression of its own direct target genes, but is also involved in signaling crosstalk with nuclear receptors such as the glucocorticoid receptor and ERα. Whether CREB1 stimulates or represses nuclear receptor activity seems to be cell-context dependent. Upon phosphorylation of serine 133 by PKA, pCREB1 can specifically recruit the coactivator CREB binding protein (CBP) and its paralog p300. The stimulatory activity of CREB1 requires its DNA binding and activation by phosphorylation, and affects the chromatin recruitment of ERα. CREB1 and ERα are biochemically associated and share hundreds to thousands of chromatin binding sites upon stimulation by estrogen and cAMP, respectively.

  • Uniprot No.：
  • 基因名：
  • 別名：
    Active transcription factor CREB antibody; cAMP response element binding protein 1 antibody; cAMP response element binding protein antibody; cAMP responsive element binding protein 1 antibody; cAMP-responsive element-binding protein 1 antibody; CREB antibody; CREB-1 antibody; CREB1 antibody; CREB1_HUMAN antibody; Cyclic AMP-responsive element-binding protein 1 antibody; MGC9284 antibody; OTTHUMP00000163864 antibody; OTTHUMP00000163865 antibody; OTTHUMP00000206660 antibody; OTTHUMP00000206662 antibody; OTTHUMP00000206667 antibody; Transactivator protein antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-CREB1 (S133)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    3C11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 功能：
    Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
  • 基因功能參考文獻：
    1. findings illustrate the significance of CREB-KDM4B-STAT3 signaling cascade in DNA damage response, and highlight that KDM4B may potentially be a novel oncotarget for colorectal cancer radiotherapy. PMID: 29633065
    2. CacyBP expression is regulated by E2F1, EGR1, and CREB transcription factors in colorectal cancer HCT116 cells. PMID: 29197151
    3. ethanol-induced eIF2alpha phosphorylation stimulates COX-2 expression and PGE2 production which induces the BACE1 expression and Abeta production via EP-2 receptor-dependent PKA/CREB pathway. PMID: 28668332
    4. Creb1/Crtc1-3 and Sec14l3 could be important for early responses of the bronchial epithelium to Th2-stimuli. PMID: 28383034
    5. CREB1 overexpression rescued the effects on gastric cancer cell growth induced by miR-1297. PMID: 29870889
    6. Results suggest that low nuclear cyclic AMP responsive element binding protein (pCREB) expression in the primary lesion is significant risk factor for metastatic melanoma. PMID: 29179997
    7. Via activation of cAMP/PKA/CREB pathway and upregulation of the downstream FtMt expression. PMID: 30069985
    8. The mechanism of prostaglandin E2-induced transcriptional up-regulation of Oncostatin-M by CREB and Sp1 has been described. PMID: 29269396
    9. The findings suggest that activation of TGR5 promoted mitochondrial biogenesis in endothelial cells, which is mediated by the CREB/PGC-1a signaling pathway. PMID: 29709472
    10. High CREB expression is associated with esophageal squamous cell carcinoma. PMID: 29286131
    11. CREB1 may activate the transcription of wtBRAF through directly binding to its promoter, increasing BRAF expression and regulating the cell proliferation, migration and invasion of endometriosis. PMID: 29286077
    12. to explore genetic variations in CREB1 promoter region and determine whether these loci affect transcriptional activity and risk on type 2 diabetes (T2D). Three polymorphisms were identified and designated as MU1, MU2 and MU3, respectively. Genotypic distribution analysis revealed that MU1 genotypes presented similar distribution between T2D and healthy controls (P>0.05) PMID: 29729382
    13. experiments mainly reveal that the CREB1 could affect glucose transport in glioma cells by regulating the expression of GLUT1, which controlled the metabolism of glioma and affected the progression of glioma. PMID: 28646353
    14. These data highlight a novel arrestin-mediated modulation of CREB signalling, suggesting a reciprocal relationship between arrestin2 and arrestin3, wherein recruitment of arrestin3 restricts the ability of beta2AR to activate prolonged CREB phosphorylation by precluding recruitment of an arrestin2/Src/p38 complex. PMID: 28733084
    15. The authors conclude that taurodeoxycholic acid-induced DNA damage may depend on the activation of TGR5, CREB and NOX5-S. It is possible that in Barrett's patients bile acids may activate NOX5-S and increase reactive oxygen species (ROS) production via activation of TGR5 and CREB. NOX5-S-derived ROS may cause DNA damage, thereby contributing to the progression from Barrett's esophagus to esophageal adenocarcinoma. PMID: 27511066
    16. The mechanism of CBP-CREB association via their pKID/KIX domains studied by molecular dynamics free energy simulations has been reported. PMID: 27054660
    17. Results indicate CREB1 as a critical transcription factor of RRM2 which promotes tumor aggressiveness, and imply a significant correlation between CREB1 and RRM2 in CRC specimens. PMID: 27801665
    18. Study suggest that both p300 and CREB are required for the function integrity of HIF-1alpha transcription machinery and subsequent angiogenesis, suggesting future studies to improve burn wound healing might be directed to optimization of the interaction between p300, CREB and HIF-1alpha. PMID: 27808477
    19. These findings suggest that CREB1 may be a potential therapeutic target for the treatment of gastric cancer PMID: 28498439
    20. YAP/ TAZ pathways contribute to the proliferation/quiescence switch during colon cancer 5FU treatment according to the concerted regulation of Cyclin E1 and CREB PMID: 27527859
    21. Data demonstrate that CREB is downregulated in glioma cells and is a direct target of miR-433-3p. These findings indicate that CREB subsequently directly or indirectly modulates its target genes to control the cell growth and metastasis in glioma. PMID: 27926502
    22. these studies demonstrate that transcription factors CREB and c-Myc maintain the transcriptional activity of STING PMID: 27835584
    23. GRK3 is a new critical activator of neuroendocrine phenotypes and mediator of CREB activation in promoting neuroendocrine differentiation of prostate cancer cells. PMID: 27191986
    24. miR-150 is a novel Wnt effector that may significantly enhance epithelial-mesenchymal transition of colorectal cancer cells by targeting the CREB signaling pathway PMID: 27285761
    25. fMRI and genotyping data from a large human sample, together with previous evidence, support the view that CREB1-associated mechanisms modulate brain function and behavior during reward-based decision-making. PMID: 26045569
    26. Knockdown of either HIF-1 or CREB or both in hypoxia reduced the expression of hypoxia-response elements- and CRE-mediated gene expression, diminished cell proliferation and increased caspase-3 activity. PMID: 27934882
    27. MnTE-2-PyP decreased p300 complex binding to a specific HRE motif within the PAI-1 gene promoter region, suppressed H3K9 acetylation, and consequently, repressed PAI-1 expression. Mechanistically, less p300 transcriptional complex binding is not due to the reduction of binding between p300 and HIF-1/CREB transcription factors, but through inhibiting the binding of HIF-1/CREB transcription factors to DNA PMID: 26944191
    28. Inhibition of CaN attenuated the hTau-induced CREB dephosphorylation with improved synapse and memory functions. PMID: 27298345
    29. Via blocking the hypoxia-mediated reduction in CREB phosphorylation. PMID: 28254846
    30. The study adds evidence that CREB, a tumor oncogene, promotes renal cell carcinoma proliferation. It probably achieves this by increasing SKA2 expression PMID: 26824422
    31. cigarette smoke extracts activate the PKA, CREB, and IL-13Ralpha2 axis in lung endothelial cells. PMID: 27986643
    32. This study showed that the induction level of IL-32 was increased in chronic rhinosinusitis with nasal polyps compared to normal nasal mucosa and that LPS-induced IL-32 expression in nasal polyp-derived fibroblasts was regulated via the TLR4/JNK/AKT/CREB signaling pathway. PMID: 27173130
    33. Studies indicate that the small molecule ICG-001 selectively blocks the cAMP response element-binding (CREB) protein (CBP)/beta-catenin or gamma-catenin interaction. PMID: 28479420
    34. Study indicates that BPA increases phosphorylated CREB in MCF-7 Cells as well as it binding to SOX2 enhancer. PMID: 28244015
    35. study concludes that miR-132 regulated SIRT1/CREB/ABCG2 signaling pathway contributing to the cisplatin resistance and might serve as a novel therapeutic target against gastric cancer PMID: 28383763
    36. Leptin also significantly increased cAMP levels, cAMP response element (CRE) activation, and CREB phosphorylation. PMID: 28571770
    37. these data show the existence of functional CREB and C/EBP binding sites in the human RIC8B gene promoter, a particular distribution of these sites and demonstrate a relevant role of CREB in stimulating transcriptional activity of this gene. PMID: 26729411
    38. MALAT1 knockdown reduces reactive gliosis, Muller cell activation, and RGC survival in vivo and in vitro MALAT1-CREB binding maintains CREB phosphorylation by inhibiting PP2A-mediated dephosphorylation, which leads to continuous CREB signaling activation. PMID: 26964565
    39. aberrant activation of CREB-C/EBPdelta axis concurring to AML onset by disrupting the myeloid cell differentiation process PMID: 27118402
    40. miR-27b-3p levels were found to be significantly negatively correlated with both NR5A2 and CREB1 levels in breast cancer tissues. PMID: 27809310
    41. Interactions between GNB3, CREB1 and negative life events were revealed. Further evidence is provided about the role of the environment in genetic vulnerability to major depression. PMID: 28225778
    42. Our study establishes a robust human stem cell-based platform for consistent quantitative evaluation of genotype-dependent Rett syndrome (RTT) phenotypes at the cellular level. PMID: 28270572
    43. UCA1 promotes cisplatin/gemcitabine resistance in bladder cancer cells through CREB modulation of miR-196a-5p expression. PMID: 27591936
    44. Report a distinct group of myxoid mesenchymal neoplasms occurring in children or young adults with a predilection for intracranial locations with EWSR1-AFT1/CREB1/CREM fusions. PMID: 28009602
    45. CREB1/FoxA1 signaling is a targetable driver of prostate cancer progression and serves as a biomarker of poor clinical outcomes. PMID: 26743006
    46. These results suggest that the HIPK2-phospho-Ser271 CREB axis is a new arsenic-responsive CREB activation mechanism in parallel with the PKA-phospho-Ser133 CREB axis. PMID: 27884605
    47. There were decreased levels of Gsa, FOXF1, CREB1, and phosphorylated CREB1 proteins in intestinal muscle layers of patients with chronic intestinal pseudo-obstruction, compared with tissues from controls. PMID: 28043906
    48. Regulatory elements for both IRF-1 (-1019 to -1016) and CREB (-1198 to -1195), specific to the distal THBS1 promoter, were required for leptin-induced TSP-1 transcription. PMID: 27281481
    49. The results suggest that Sirt2 plays a crucial role in neuronal differentiation via the ERK-CREB signaling pathway. PMID: 27838300
    50. revealed more than 170 NFAT-associated proteins, half of which are involved in transcriptional regulation. Among them are many hitherto unknown interaction partners of NFATc1 and NFATc2 in T cells, such as Raptor, CHEK1, CREB1, RUNX1, SATB1, Ikaros, and Helios. PMID: 27637333

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  • 相關(guān)疾?。?/div>
    Angiomatoid fibrous histiocytoma (AFH)
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2345

    OMIM: 123810

    KEGG: hsa:1385

    STRING: 9606.ENSP00000387699

    UniGene: Hs.516646