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  4. AIFM1 Recombinant Monoclonal Antibody

AIFM1 Recombinant Monoclonal Antibody

  • 中文名稱：
    AIFM1重組抗體
  • 貨號：
    CSB-RA296617A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Rat liver tissue, Rat kidney tissue
      All lanes: AIFM1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 67, 36, 29, 27 kDa
      Observed band size: 67 kDa
    • IHC image of CSB-RA296617A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA296617A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    AIFM1 antibody CSB-RA296617A0HU is a recombinant monoclonal antibody produced from the expression of the plasmids that were constructed by the AIFM1 monoclonal antibody (generated from animals with the human AIFM1 synthesized peptide immunization) DNA sequence in cell lines. This AIFM1 recombinant antibody is a rabbit IgG antibody. It was purified using the affinity-chromatography method. It can recognize and detect the AIFM1 protein from human and rat samples. And it is suitable for ELISA, IHC, and WB analyses.

    The target protein AIFM1 is released from the mitochondrial intermembrane space (IMS) during apoptosis and can induce chromatin condensation and DNA fragmentation in a caspase-independent manner. It also functions as a mitochondrial flavin adenine dinucleotide (FAD)-dependent nicotinamide adenine dinucleotide (NADH) oxidoreductase that regulates respiratory complex assembly and function, produces reactive oxygen species (ROS), and coordinates parthanatos.

  • Uniprot No.：
  • 基因名：
  • 別名：
    Apoptosis-inducing factor 1, mitochondrial (EC 1.1.1.-) (Programmed cell death protein 8), AIFM1, AIF PDCD8
  • 反應(yīng)種屬：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human AIF
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    4B2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 功能：
    Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import.; Has NADH oxidoreductase activity. Does not induce nuclear apoptosis.; Pro-apoptotic isoform.
  • 基因功能參考文獻(xiàn)：
    1. Here, the binding site of AIF(Delta1-121) and AIF(370-394) on CypA has been mapped by NMR spectroscopy and biochemical studies, and a molecular model of the complex has been proposed. The authors show that AIF(370-394) interacts with CypA on the same surface recognized by AIF(Delta1-121) protein and that the region is very close to the CypA catalytic pocket. PMID: 28442737
    2. new form of motor neuropathy expands the phenotypic spectrum of AIFM1 mutations and therefore, the AIFM1 gene should be considered in the diagnosis of hereditary motor neuropathies PMID: 28975462
    3. X-linked hypomyelination with spondylometaphyseal dysplasia associated with mutations in AIFM1 PMID: 28842795
    4. report of a family carrying a novel missense mutation in AIFM1 that exhibits an isolated axonal polyneuropathy with misassembly of mitochondrial complex I and III. PMID: 28888069
    5. we sought to investigate mechanisms mediated by Hsp70 acetylation in relation to apoptotic and autophagic programmed cell death. Upon stress-induced apoptosis, Hsp70 acetylation inhibits apoptotic cell death, mediated by Hsp70 association with apoptotic protease-activating factor (Apaf)-1 and apoptosis-inducing factor (AIF), key modulators of caspase-dependent and -independent apoptotic pathways, respectively PMID: 28627586
    6. Overexpression of full-length AIFM1 suppresses proliferation and induces apoptosis of HepG2 and Hep3B cells. Caspase 3 and DRAM are involved in full-length AIFM1-induced apoptosis in HepG2 and Hep3B cells. PMID: 29501488
    7. Real-time PCR results showed that apoptosis-inducing factor (AIF) and calpain-1 mRNAs were highly expressed in the transitional segmentof the Hirschsprung's disease bowel, whereas autophagy protein 5 (Atg5) was highly expressed in the narrow segment. Western blot results were consistent with mRNA levels, with increased AIF, calpain-1, and Atg5 expressions in the transitional segment compared with the dilated segment. PMID: 28562483
    8. Data revealed that under a nutrient deficient condition, CD317 functions as an anti-apoptotic factor through AIF-mediated caspase and autophagy-independent manner. PMID: 27444183
    9. we report that AIF-independent PARP-1-dependent necrosis constitutes a major mechanism of RPE cell death leading to retinal degeneration in dry age-related macular degeneration . PMID: 28055012
    10. Downregulation of AIF by hypoxia causes oxidative inactivation of the lipid phosphatase activity of phosphatase and tensin homolog on chromosome 10 (PTEN), with ensuing activation of Akt kinase, phosphorylation of the Akt substrate GSK-3beta and activation of WNT/beta-catenin signaling in colon cancer cells. PMID: 27543779
    11. novel pathway for mda-7/IL-24-induced caspase-independent apoptosis in neuroblastoma cells mediated through modulation of AIF, ATM, and gamma-H2AX. PMID: 27197168
    12. Study defines the mechanism whereby AIF is allosterically modulated by charge-complex formation with NADH. Two distinct molecular pathways couple AIF's active site to dimerization and release of its regulatory C-loop insert with implications for AIF allosteric switching in cell survival and death. PMID: 27818101
    13. Whole exome sequencing indicated a single plausible candidate - the p.Asp237Gly variant in apoptosis inducing factor mitochondria associated 1 (AIFM1; chr. Xq26.1), and the p.Asp237Gly segregated with disease in two families. PMID: 27102849
    14. Only a drastic decrease in the expression level or/and redox activity of AIF tends to cause an early and severe neurodegeneration, whereas less pronounced changes in the AIF properties could lead to a broad range of slowly progressive neurological disorders. PMID: 27178839
    15. AIF translocation plays a role in influenza-virus-induced apoptosis. PMID: 27853862
    16. Copper induces the activation of apoptosis through casp3/casp8/casp9/AIF/TP53 signaling pathways. Suggest that apoptosis activation mechanism is dependent on the concentration, time of exposure to Cu and cell type. PMID: 27046389
    17. Estradiol inhibits oxidative stress-induced accumulation of AIF in nucleolus and PARP1-dependent cell death via estrogen receptor alpha in MCF-7 cells. PMID: 25280774
    18. AIF may participate in but not be dominant in the process of retinal pigment epithelium cell apoptosis induced by oxidative stress. PMID: 27453270
    19. Altogether these data indicate that AIF supports the growth and survival of metabolically defined pancreatic cancer cells and that this metabolic function may derive from a novel mechanism so far undocumented in other cancer types. PMID: 27108222
    20. Cdk5-mediated phosphorylation of CHIP negatively regulates its neuroprotective function, thereby contributing to truncated AIF mediated neuronal cell death progression following neurotoxic stimuli. PMID: 26206088
    21. Results identify PTEN as the substrate of AIF oxidoreductase and reveal a novel function for AIF in controlling tumor metastasis. PMID: 26415504
    22. UDA decreased the St-induced changes in mitochondrial and cytosolic AIF level, whereas in Dox-model it affected only the cytosolic AIF content. PMID: 26012840
    23. strongly support a direct relationship between AIF nuclear translocation and radiation induced cell death in hepatocellular carcinoma cell line HepG2 PMID: 26920061
    24. identification of the novel interaction between Trx1 and AIF has provided opportunities to design and develop therapeutically relevant strategies that either promote or prevent this protein-protein interaction for the treatment of different disease PMID: 26119781
    25. AIF bears a HSP-70 binding domain allowing association with HSP-70. PMID: 26165468
    26. Missense mutations in AIFM1 are associated with X-linked recessive auditory neuropathy spectrum disorder. PMID: 25986071
    27. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type). PMID: 26063804
    28. Summarizing what is known today, mutations in AIFM1 are associated with a progressive disorder with myopathy, ataxia and neuropathy. PMID: 25583628
    29. These results suggest that AIF downregulation is a common event in kidney tumor development. AIF loss may lead to decreased STK3 activity, defective apoptosis and malignant transformation PMID: 24992339
    30. Residues Regulating the Reductase Activity of the Human Mitochondrial Apoptosis Inducing Factor PMID: 26237213
    31. Data indicate that poptosis-inducing factor (AIF) controls the mitochondrial import of mitochondrial membrane transport protein CHCHD4. PMID: 26004228
    32. review describes the multistep course of parthanatos and its dependence on PAR signalling and nuclear AIF translocation. PMID: 24684389
    33. Increased trophoblast apoptosis and altered expression levels of syncytin-1, calpain 1, and AIF is observed in preeclamptic placentas. PMID: 24413738
    34. Inhibition of the AIF/CypA complex protects against intrinsic death pathways induced by oxidative stress. PMID: 24434516
    35. both the mitochondrial and apoptotic functions of hAIF are interconnected and coenzyme controlled: a key information in the understanding of the physiological role of AIF in the cellular life and death cycle. PMID: 24914854
    36. The E1B-55K or E4orf3 proteins independently prevented nuclear fragmentation subsequent to release of AIF from the mitochondria, possibly by altering the intracellular distribution of PAR-modified proteins. PMID: 24889240
    37. Data show that buthionine sulfoximine and synergizes with hydroxychavicol in inducing apoptosis of chronic myeloid leukemic (CML) cells through the GSH-ROS-JNK-ERK-iNOS pathway by apoptosis inducing factor (AIF)-dependent manner. PMID: 24040019
    38. Aif seems to have pro-apoptotic and anti-apoptotic roles in the same lineage in different contexts and at different stages PMID: 24434313
    39. Bit1 plays pivotal roles in the development and progression of ESCC, and its biological functions in ESCC may be closely associated with AIF and Bcl-2 levels. PMID: 23955799
    40. Apoptosis-inducing factor and leukocyte elastase inhibitor derived DNase II interact and can cooperate to induce cell death. PMID: 23673989
    41. The diarylheptanoid hirsutenone sensitizes chemoresistant ovarian cancer cells to cisplatin via modulation of apoptosis-inducing factor and X-linked inhibitor of apoptosis. PMID: 24247248
    42. the caspase-independent mitochondrial apoptosis factor, apoptosis-inducing factor (AIF) was upregulated following SPG treatment. These findings indicate that SPG exerts its anticancer effects on RC-58T/h/SA#4 primary prostate cancer cells PMID: 23443329
    43. Calpain activation via Ca(2) flux plays an essential role in eliciting an AIF-mediated, caspase-independent apoptotic pathway in EV71-infected cells. PMID: 23515028
    44. AIF knockdown disrupts function of complex I and therefore increases the superoxide production in mitochondria PMID: 23682427
    45. Taken together our results demonstrate that parthenolide causes in the two cell lines a caspase-independent cell death, which is mediated by AIF(apoptosis-inducing factor ). PMID: 22688575
    46. AIF is an important factor for advanced prostate cancer cells and that through control of energy metabolism and redox balance, the enzymatic activity of AIF is critical for this support. PMID: 23118229
    47. Our findings expand the spectrum of AIF-related disease and provide insight into the effects of AIFM1 mutations. PMID: 23217327
    48. We found AIF to be strongly expressed in the villous trophoblast and that small amounts of AIF were localized to the nucleus of the cells. PMID: 22133578
    49. AIF is imported from the endoplasmic reticulum to the mitochondria via mitochondria-associated membranes and transport vesicles. PMID: 22134679
    50. Different mechanisms preserve translation of programmed cell death 8 and JunB in poliovirus-infected endothelial cells. PMID: 22328780

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  • 相關(guān)疾?。?/div>
    Combined oxidative phosphorylation deficiency 6 (COXPD6); Cowchock syndrome (COWCK); Deafness, X-linked, 5 (DFNX5)
  • 亞細(xì)胞定位：
    Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region.; [Isoform 3]: Mitochondrion intermembrane space. Mitochondrion inner membrane.; [Isoform 4]: Mitochondrion. Cytoplasm, cytosol.; [Isoform 5]: Cytoplasm.
  • 蛋白家族：
    FAD-dependent oxidoreductase family
  • 組織特異性：
    Expressed in all tested tissues. Detected in muscle and skin fibroblasts (at protein level). Expressed in osteoblasts (at protein level).; [Isoform 3]: Brain specific.; [Isoform 4]: Expressed in all tested tissues except brain.; [Isoform 5]: Isoform 5 is
  • 數(shù)據(jù)庫鏈接：

    HGNC: 8768

    OMIM: 300169

    KEGG: hsa:9131

    STRING: 9606.ENSP00000287295

    UniGene: Hs.424932