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PPM1D Antibody

  • 中文名稱：
    PPM1D兔多克隆抗體
  • 貨號：
    CSB-PA018491ESR1HU
  • 規(guī)格：
    ￥440
  • 促銷：
    小規(guī)格抗體限時一口價
  • 圖片：
    • Western blot
      All lanes: PPM1Dantibody at 2.36μg/ml
      Lane 1: Rat gonadal tissue
      Lane 2: U87 whole cell lysate
      Secondary
      Goat polyclonal to rabbit IgG at 1/10000 dilution
      Predicted band size: 67, 48 kDa
      Observed band size: 67 kDa
    • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA018491ESR1HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA018491ESR1HU at dilution of 1:100
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) PPM1D Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    EC 3.1.3.16 antibody; p53 induced protein phosphatase 1 antibody; p53-induced protein phosphatase 1 antibody; PP2C delta antibody; PP2C-delta antibody; PP2CD antibody; Ppm1d antibody; PPM1D_HUMAN antibody; Protein phosphatase 1D antibody; protein phosphatase 1D magnesium-dependent delta isoform antibody; Protein phosphatase 2C delta isoform antibody; Protein phosphatase 2C isoform delta antibody; Protein phosphatase magnesium dependent 1 delta antibody; Protein phosphatase magnesium-dependent 1 delta antibody; Protein phosphatase Mg2+/Mn2+ dependent 1D antibody; Protein phosphatase Wip1 antibody; Wild type p53 induced phosphatase 1 antibody; WIP 1 antibody; WIP1 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Rat
  • 免疫原：
    Recombinant Human Protein phosphatase 1D protein (1-250AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.
  • 基因功能參考文獻：
    1. The observations suggest a novel role of ATR kinase in mediating its own signal attenuation via PPM1D recruitment to chromatin as an essential mechanism for restarting the stalled forks, cell cycle re-entry and cellular recovery from replication stress. PMID: 29485113
    2. NF-kappaB served as a positive transcriptional regulator of WIP1 to activate its expression and affect its function in colorectal cancer cells. PMID: 29367109
    3. ese results enhance our understanding of the molecular mechanisms that govern nucleoli formation by demonstrating that PPM1D regulates nucleolar formation by regulating NPM phosphorylation status through a novel signalling pathway, PPM1D-CDC25C-CDK1-PLK1 PMID: 27619510
    4. These data support the notion that Wip1 contributes to the formation of the ERMs in PDR membranes via NF-kappaB signaling. PMID: 28402943
    5. In an in vitro model of the blood-brain barrier, LPS raised Wip1 expression. Wip1 knockdown increased permeability and decreased transepithelial electrical resistance, protein expression of ZO-1, and occluding. Wip1 silencing augmented TNF-alpha, IL-1beta, IL-12, and IL-6. Sonic hedgehog signaling was activated by Wip1 overexpression and inhibited by silencing. Wip1 may protect the BBB against LPS damage via SHH signal... PMID: 29128669
    6. Studies suugest Wip1 role in tumorigenesis through regulation of p53 and p38MAPK pathways. PMID: 26883196
    7. The inhibition of Wip1 might fortify p53-mediated tumor suppression by Mdm2 antagonists. PMID: 27183917
    8. Wip1 suppressed ovarian cancer cell invasion, migration, epithelial to mesenchymal transition (EMT), and ovarian cancer metastasis in xenograft animal models. PMID: 27121065
    9. model reproduces the observed cellular phenotypes in experiments: oscillatory (for low DNA damage) regulated by negative feedback loops involving mainly p53 and Mdm2 and apoptotic or senescent (for high DNA damage) regulated by the positive p53/Wip1/miR-16 feedback loop PMID: 28968438
    10. This suggests an important cross-talk between SHH and WIP1 pathways that accelerates tumorigenesis and supports WIP1 inhibition as a potential treatment strategy for MB. PMID: 27086929
    11. Wip1 activity and its relevance to cancer as an oncoprotein is reviewed PMID: 28385459
    12. The authors show that a global spread of ATM activity on chromatin and phosphorylation of ATM targets including KAP1 control Plk1 re-activation. These phosphorylations are rapidly counteracted by the chromatin-bound phosphatase Wip1, allowing cell cycle restart despite persistent ATM activity present at DNA lesions. PMID: 28607002
    13. Truncating and missense PPM1D mutations are associated with prostate cancer. PMID: 27401275
    14. this study shows that expression of Wip1 is decreased in patients with active inflammatory bowel disease PMID: 27687530
    15. Wip1 is frequently overexpressed in nonsmall cell lung cancer, which may serve an essential role in the p38MAPK/p53/p16 signaling pathway. PMID: 27959454
    16. Study identified 14 individuals with mild to severe Intellectual Disability (ID) and/or developmental delay and de novo truncating PPM1D mutations; all mutations were located in the last or penultimate exon, suggesting escape from nonsense-mediated mRNA decay. PMID: 28343630
    17. PPM1D gene silencing combined with TMZ eradicates glioma cells through cell apoptosis and cell cycle arrest PMID: 27633132
    18. High PPM1D expression is associated with breast cancer. PMID: 27485113
    19. PPM1D in the chromosomal location 17q22-24 might be the most relevant candidate gene with respect to a potential functional implication in meningioma progression. PMID: 26942600
    20. potential prognostic marker and therapeutic target for hepatocellular carcinoma PMID: 26809466
    21. PPM1D suppresses pancreatic cancer cell apoptosis via inhibition of the p38 MAPK/p53 pathway through both dephosphorylation of p38 MAPK and downregulation of ASPP2. PMID: 26714478
    22. squalene inhibits the ATM-dependent signaling pathway following DNA damage through intracellular induction of Wip1 expression. PMID: 26824362
    23. Chemotherapy exposure and age influence the accumulation of PPM1D mutations in the peripheral blood mononuclear cells of ovarian cancer patients. PMID: 26847329
    24. Knockdown of Wip1 enhances sensitivity to radiation in HeLa cells by inhibiting cell proliferation and inducing apoptosis through activation of p38 MAPK. PMID: 26351212
    25. Results showed evidence that Wip1 underwent Cdh1-dependent proteolysis during mitosis and sustained Wip1 activity during mitosis, resulting in mitotic delay at the metaphase to anaphase transition. PMID: 25649870
    26. Study reveals a mechanism of stem cell aging, in which distinct effects of p53 and mTORC1 pathways on HSC aging are governed by Wip1. PMID: 25879755
    27. WIP1 silencing reduced expression of MMP-9, VEGF-C, cyclin D1, and c-Myc, as well as migration and invasion of salivary adenoid cystic carcinoma cells. WIP1 expression was positively associated with ACC metastasis and prognosis. PMID: 25797250
    28. Wip1 may be involved in the biological processes of nasopharyngeal cancer cell proliferation, apoptosis, and migration and invasion. PMID: 25060857
    29. p21 might contribute to positive regulation of WIP1, resulting in dephosphorylation of the histone H2A variant gammaH2AX. PMID: 26006237
    30. PPM1D plays an important role in promoting CDDP resistance. PMID: 25154814
    31. data suggests that WIP1 inhibition represents a novel therapeutic approach to neuroblastoma that could be integrated with current chemotherapeutic approaches PMID: 25658463
    32. ATM kinase and Wip1 phosphatase were identified as opposing regulators of DAXX-S564 phosphorylation and the role of DAXX phosphorylation and DAXX itself are independent of p53-mediated gene expression. PMID: 25659035
    33. Wip1 protein was increased in kidney carcinoma. PMID: 24970693
    34. Results show that downregulation of HIPK2 increases resistance of bladder cancer cell to cisplatin by regulating Wip1. PMID: 24846322
    35. PPM1D plays an important role in the progression of NSCLC. PMID: 25412952
    36. High PPM1D expression is associated with lung cancer. PMID: 25123458
    37. The truncating mutations of PPM1D are present in blood DNA of NSCLC patients. PMID: 25742468
    38. Results demonstrate increased WIP1 expression in metastatic medulloblastomas, and inferior survival in patients with WIP1 high-expressing medulloblastoma. PMID: 24632620
    39. WIP1 knockdown inhibited the invasiveness of CNE-2 and 5-8F cells and was associated with the down-regulation of the expression of matrix metallopeptidase 9 PMID: 24801909
    40. Gain-of-function PPM1D mutations are associated with brainstem gliomas. PMID: 24880341
    41. High PPM1D expression is associated with prostate cancer. PMID: 24922655
    42. PP4 and Wip1 are differentially required to counteract the p53-dependent cell cycle arrest in G1 and G2, by antagonizing early or late p53-mediated responses, respectively PMID: 24711418
    43. Wip1 may be involved in the biological processes of non small cell lung cancer cell proliferation, cell apoptosis, and cell cycle. PMID: 24272082
    44. PPM1D plays a crucial role in colorectal carcinoma cell proliferation and colony formation. PMID: 23745790
    45. PPM1D mutations in the mosaic state predispose women to breast and ovarian cancer in the absence of a family history of cancer PMID: 24262437
    46. Expression of Wip1 in neutrophils was downregulated quickly after neutrophil activation through JNK-microRNA-16 pathway. It was negatively correlated with inflammatory cytokine productions of neutrophils in sepsis patients. PMID: 24395919
    47. Genetic variants and mutations of PPM1D control the response to DNA damage. PMID: 23907125
    48. BAX dephosphorylation of Wip1 phosphatase is an important regulator of resistance to anticancer therapy. PMID: 23907458
    49. High WIP1 expression is associated with colorectal cancer. PMID: 23679302
    50. Wip1 plays an important role in the regulation of global heterochromatin silencing and thus is critical in maintaining genome integrity PMID: 24135283

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  • 相關疾?。?/div>
    Intellectual developmental disorder with gastrointestinal difficulties and high pain threshold (IDDGIP); Breast cancer (BC); Ovarian cancer (OC)
  • 亞細胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    PP2C family
  • 組織特異性：
    Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9277

    OMIM: 114480

    KEGG: hsa:8493

    STRING: 9606.ENSP00000306682

    UniGene: Hs.286073