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  4. Rat Acetylcholinesterase,AChE ELISA Kit

Rat Acetylcholinesterase,AChE ELISA Kit

  • 中文名稱：
    大鼠乙酰膽堿酯酶(AChE)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11304r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Rat ACHE ELISA Kit was designed for the quantitative measurement of Rat ACHE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .

  • 別名：
    AcheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %92
    Range %88-95
    1:200Average %96
    Range %90-101
    1:400Average %94
    Range %90-97
    1:800Average %99
    Range %95-103
  • 回收率：
    The recovery of rat AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-99
    EDTA plasma (n=4)9287-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.361 2.384 2.373 2.232
    2501.636 1.658 1.647 1.506
    1251.044 1.057 1.051 0.910
    62.50.610 0.599 0.605 0.464
    31.20.413 0.404 0.409 0.268
    15.60.305 0.297 0.301 0.160
    7.80.237 0.228 0.233 0.092
    00.142 0.140 0.141 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
  • 基因功能參考文獻：
    1. sepsis decreased AChE activity by reducing its expression at the neuromuscular junction. PMID: 28849127
    2. Paraquat-induced death of hippocampal neurons was partially mediated by AChE variants inducing and cholinergic and gultamatergic transmission disruption. PMID: 28916328
    3. Quercetin improves memory and anxiogenic-like behavior in diabetic rats by preventing the increase in Ache activity seen in streptozocin induced diabetes. PMID: 27694000
    4. the induction of PRiMA-linked AChE in osteoblast should be independent to classical estrogen signaling pathway. PMID: 27019979
    5. This study showed that changes in AChE may, at least in part, to lead to memory impairment caused by galactose. Taken together, our results can help understand the etiopathology of classical galactosemia. PMID: 26948151
    6. Acetylcholinesterase activity was increased in rats subjected to mild hyperhomocysteinemia PMID: 24590316
    7. Hindlimb unloading (simulation of hypogravity) leads to an increase in functional activity of acetylcholinesterase on the background of reduced postsynaptic membrane area occupied by acetylcholine receptors. PMID: 25711085
    8. Under all studied experimental conditions, offspring brain acetylcholinesterase (AChE) activity was found to be significantly decreased due to maternal hypothyroidism PMID: 24632022
    9. AChE has an important role in modulation of early immune responses against Toxoplasma gondii infection. PMID: 24039284
    10. Spindle-shaped AChE-rich cells in the floccular stalk project to the flocculus, nodulus and uvula, but not to the cerebellar hemispheres. PMID: 24010172
    11. Tyrosine increased acetylcholinesterase in blood & all tested brain areas. Acute administration decreased its mRNA levels in hippocampus. Chronic administration did the same in the hippocampus. PMID: 23046746
    12. Acetylcholinesterase inhibitor huperazine A improved, or partly reversed, the Abeta-induced damage of neurite outgrowth. PMID: 23119107
    13. in the absence of pituitary growth hormone the postnatal proliferation of cholinergic synapses in the rat spinal cord where AChE activity is abundant, is markedly reduced PMID: 22922167
    14. Hypothyroidism significantly reduced both specific and total AChE activity in spinal cord of rat pups PMID: 22982053
    15. The results from the present study demonstrate a marked increase in AChE activity in all brain structures following the administration of a branched-chain amino acid pool. PMID: 22328136
    16. This study demonistrated that AChE positive neurons were stained lightly, then during later developmental periods the staining gradually increased and achieved adult level of intensity on the day P21 in spinal cord. PMID: 22028090
    17. During induction of the non-alcoholic fatty liver disease with CCl4 AChE activity was significantly increased compared to control in blood, liver and brain tissues. PMID: 21881966
    18. AChE activity can act as purinergic and cholinergic markers in lymphocytes PMID: 20832780
    19. effect of melatonin on neuroinflammation and acetylcholinesterase activity induced by LPS in rat brain PMID: 20450904
    20. These findings suggest that the restricted localization of PRiMA-linked G4 AChE at the nmjs could be contributed by the pre-synaptic motor neuron and/or the post-synaptic muscle fiber. PMID: 19680821
    21. the importance of post-transcriptional mechanisms in regulating AChE expression in differentiating neurons and implicate HuD as a key trans-acting factor in these events PMID: 12468554
    22. The activity of this protein is completely suppressed by the administration of LD50 of phosphacol. PMID: 12629810
    23. AChE is necessary for normal dendrite and axon formation in hippocampal neurones and suggest that it may also play a role in excitatory synapse development, plasticity, and remodelling. PMID: 12799140
    24. Local increment in neuronal AChE concentration in the mammalian hippocampus, such as in amyloid deposits, may play a role in triggering neuropathological and behavioral changes such as those observed in Alzheimer disease brains PMID: 12969266
    25. In this study we show an effect of AChE that could contribute to the increased deposition of Amyloid beta PMID: 14678761
    26. AChE is involved in regulating cell-matrix interactions in bone PMID: 15454088
    27. Nearly all AChE-immunoreactive neurons in the Lateral hypothalamus express immunoreactivity for either melanin concentrating hormone or for orexin PMID: 15488307
    28. rats subjected to ovariectomy presented a significant increase in brain Na+, K+-ATPase, AChE and CAT activities, but did not change the oxidative stress parameters PMID: 15869839
    29. Results suggest that the presence of acetylcholinesterase protein doesn't correlate with catalytic activity, and the diffuse cytoplasmic and plasma membrane localization of AChE is a property of neuronal cell types. PMID: 16052514
    30. action of P2Y1 receptor could lead Ache gene activation in cultured cortical neurons PMID: 16429571
    31. Lead acetate decreased brain AChE activity of rats in dose/age dependent manner. PMID: 16678265
    32. The SH-amino acids seem to affect the hippocampal enzyme indirectly, possibly by lipid(s)-protein modifications(s) and Hci by inducing oxidative stress, since no effect was observed on pure AChE activity PMID: 16773467
    33. Results suggest that interferon-beta may act through inhibition of acetylcholinesterase, and ethidium bromide could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions. PMID: 16871442
    34. In the cerebellum, hyperthyroidism provoked a significant decrease in both the AChE and the Na(+), K(+)-ATPase activities PMID: 17165152
    35. findings provide evidence for intra- and supramodal influences of the neonatal removal of retinal input on neural activity- and use-dependent modifications of cortical AChE activity PMID: 17280650
    36. In choline-deprived (CD) newborn rats,ANTIOXIDANT STATUS and AChE and Na(+),K(+)-ATPase activities were significantly reduced by 23%, 24% and 50%, respectively, in the brains of both sexes. PMID: 17484629
    37. Low concentrations of aspartame metabolites had no effect on AChE activity, whereas high or toxic concentrations decreased enzyme activity. PMID: 17580119
    38. that the 55-kDa AChE may be involved in different biological processes such as neural development, tumor progression, and angiogenesis. PMID: 17606622
    39. These findings suggest that cell surface AChE, possibly in a novel signalling complex containing APP and perlecan, contributes to a generalised mechanism for polarised membrane protrusion and migration in all adherent cells. PMID: 17948252
    40. Repeated exposure to low dose malathion increased brain AChE activity and altered antioxidant levels. PMID: 18341513
    41. Data show that acetylcholine esterase enzyme activity shows a significant decrease in cerebral cortex and a increase in the muscle of experimental hypoxic neonatal rats. PMID: 18406032
    42. Although the mechanisms of action of GAA on AChE activity and on memory are unclear, these findings suggest that the accumulation of GAA found in patients with GAMT-deficiency may be one of the mechanisms involved in neural dysfunction. PMID: 18437545
    43. Tanshinone modulates AChE and NOS proteins concentrations in the hippocampus of AD rats. This may have therapeutic potential in AD rats. PMID: 18980714
    44. Protein CutA undergoes an unusual transfer into the secretory pathway and affects the folding, oligomerization, and secretion of acetylcholinesterase. PMID: 19049969
    45. cell death due to paraoxon not AChE dependent PMID: 19378919
    46. Data show that after denervation, the expression of PRiMA, AChE(T) and G(4) AChE decreased markedly in the spinal cord, and in fast- and slow-twitch muscles. PMID: 19490106

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  • 亞細胞定位：
    Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein.; [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 組織特異性：
    Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver.
  • 數(shù)據(jù)庫鏈接：

    KEGG: rno:83817

    UniGene: Rn.105879