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  4. Mouse diamine oxidase,DAO ELISA Kit

Mouse diamine oxidase,DAO ELISA Kit

  • 中文名稱：
    小鼠二胺氧化酶(DAO)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E10090m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：

    This Mouse DAO ELISA Kit was designed for the quantitative measurement of Mouse DAO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 mU/mL-1000 mU/mL and the sensitivity is 3.9 mU/mL.

  • 別名：
    Aoc1 ELISA kit; Abp1Amiloride-sensitive amine oxidase [copper-containing] ELISA kit; DAO ELISA kit; Diamine oxidase ELISA kit; EC 1.4.3.22 ELISA kit; Amiloride-binding protein 1 ELISA kit; Amine oxidase copper domain-containing protein 1 ELISA kit; Histaminase ELISA kit
  • 縮寫：
    ABP1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 mU/mL-1000 mU/mL
  • 靈敏度：
    3.9 mU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %81-87
    1:2Average %95
    Range %91-99
    1:4Average %91
    Range %88-96
    1:8Average %96
    Range %93-99
  • 回收率：
    The recovery of mouse DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 109104-112
    EDTA plasma (n=4)8581-89
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    10002.879 2.752 2.816 2.607
    5002.301 2.216 2.259 2.050
    2501.584 1.560 1.572 1.363
    1250.945 0.932 0.939 0.730
    62.50.655 0.672 0.664 0.455
    31.20.532 0.542 0.537 0.328
    15.60.412 0.407 0.410 0.201
    00.211 0.206 0.209
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse DAO antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled DAO antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

問答及客戶評論

■ 常見問題解答
Q:

小鼠DAO ELISA試劑盒使用什么稀釋血清?

A:
我們4倍稀釋檢測血清和血漿，這僅供參考。不同的樣品采集時間不同，檢測和稀釋條件也會有所不同。我們建議您先做一個預測試，以確定最佳稀釋倍數(shù)。

靶點詳情

  • 功能：
    Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis.
  • 亞細胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Copper/topaquinone oxidase family
  • 數(shù)據庫鏈接：