Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

Mouse coagulation factor Ⅶ,FⅦ ELISA Kit

  • 中文名稱：
    小鼠凝血因子Ⅶ(FⅦ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17817m
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse Coagulation factor VII (F7) ELISA Kit was designed for the quantitative measurement of Mouse Coagulation factor VII (F7) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.

  • 別名：
    F7 ELISA Kit; Cf7 ELISA Kit; Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.45 ng/mL-30 ng/mL
  • 靈敏度：
    0.11 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Blood Coagulation
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FⅦ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1000 Average % 92    
    Range % 86-96    
    1:2000 Average % 97    
    Range % 92-102    
    1:4000 Average % 93    
    Range % 88-97    
    1:8000 Average % 89    
    Range % 84-94    
  • 回收率：
    The recovery of mouse FⅦ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 88-95    
    EDTA plasma (n=4) 108 103-114    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    30 2.468 2.445 2.457 2.324    
    15 2.115 1.951 2.033 1.900    
    7.5 1.503 1.440 1.472 1.339    
    3.75 0.845 0.790 0.818 0.685    
    1.8 0.626 0.593 0.610 0.477    
    0.9 0.381 0.362 0.372 0.239    
    0.45 0.274 0.252 0.263 0.130    
    0 0.137 0.129 0.133 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • 基因功能參考文獻：
    1. Leu8 is crucial for FVIIa-EPCR binding; this study characterizes its interaction in vivo in mice PMID: 28035745
    2. FVIIa-antithrombin but not FVIIa is a ligand for LRP1, and LRP1 contributes to the clearance of FVIIa-antithrombin in vivo PMID: 27614059
    3. FVIIa binding to EPCR leads to a barrier protective effect in vivo PMID: 24977291
    4. Murine FVIIa binds poorly to murine EPCR. PMID: 22370814
    5. GlycoPEGylated rFVIIa (N7-GP) has a prolonged hemostatic effect in hemophilic mice compared with rFVIIa PMID: 21366858
    6. Conclude that the fVII-targeted verteporfin photodynamic therapy that we report here is a novel and effective therapeutic with improved selectivity for the treatment of breast cancer. PMID: 20504328
    7. Recombinant FVIIa readily associates with the vascular endothelium and subsequently enters into extravascular spaces where it is likely to bind to tissue factor and is retained for extended time periods. PMID: 19943873
    8. true circadian rhythms for FVII were found PMID: 15604416
    9. Data suggest that long-term expression of murine activated factor VII is safe, but elevated levels cause premature mortality. PMID: 18398505
    10. FVIIa functions in the asthmatic response to an allergen by stimulating lung eosinophilia, airway hyperresponsiveness, and mucin production. PMID: 19286924
    11. Plasma elimination kinetics for factor VII are independent of its activation to factor VIIa and complex formation with plasma inhibitors. PMID: 19404533

    顯示更多

    收起更多

  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Plasma and liver.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:14068

    STRING: 10090.ENSMUSP00000033820

    UniGene: Mm.4827