Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT
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中文名稱:小鼠血管內(nèi)皮細(xì)胞生長因子(VEGF)酶聯(lián)免疫試劑盒
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貨號:CSB-E04756m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:VEGFA(血管內(nèi)皮生長因子A)是一種在人體中發(fā)揮重要作用的細(xì)胞因子,它促進(jìn)血管新生和修復(fù),并參與了許多生物學(xué)過程,如器官發(fā)育和腫瘤發(fā)生等。VEGFA在腫瘤治療中也有重要的應(yīng)用,如抑制血管生成來抑制腫瘤生長。華美生物所提供的Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT屬于ELISA檢測試劑盒,采用雙抗夾心法定量檢測鼠血清、血漿、細(xì)胞培養(yǎng)上清、組織勻漿樣本中的VEGFA,其靈敏度為0.857 pg/mL,檢測范圍為3.906 pg/mL-250 pg/mL。
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別名:Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:3.906 pg/mL-250 pg/mL
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靈敏度:0.857 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cancer
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of Mouse VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Therapeutic Role of Low Doses of Gamma Irradiation on Ehrlich Carcinoma Bearing Mice RS Abd Allah,Egyptian Academic Journal of Biological Sciences(B.Zoology),2024
- Comparison of two hemostatic skin adhesive dressings, incorporating multi-metal bioactive glass M Ghasemian,Engineered Regeneration,2024
- Biodegradable Cardiac Occluder with Surface Modification by Gelatin–Peptide Conjugate to Promote Endogenous Tissue Regeneration P Kong,Advanced science,2024
- Targeted modulation of intestinal epithelial regeneration and immune response in ulcerative colitis using dual-targeting bilirubin nanoparticles L Pervizaj-Oruqaj,Theranostics,2024
- Single-cell analysis reveals inflammatory interactions driving macular degeneration M Kuchroo,Nature communications,2023
- Atf4 regulates angiogenic differences between alveolar bone and long bone macrophages by regulating M1 polarization, based on single-cell RNA sequencing, RNA-seq and ATAC-seq analysis L Gu,Journal of translational medicine,2023
- SIRT1/FOXO1 Axis-Mediated Hippocampal Angiogenesis is Involved in the Antidepressant Effect of Chaihu Shugan San S Zhang,Drug design, development and therapy,2022
- Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production D Pan,Cellular signalling,2022
- STAT3 activation by catalpol promotes osteogenesis-angiogenesis coupling, thus accelerating osteoporotic bone repair Yating Li,Stem Cell Research & Therapy,2021
- Construction of biological factor-coated stent and its effect on promoting endothelialization Xiuhong Huang,Materials Science & Engineering C-Materials for Biological Applications,2021
- Recapitulation of In Situ Endochondral Ossification Using an Injectable Hypoxia-Mimetic Hydrogel L Sun,ADVANCED FUNCTIONAL MATERIALS,2020
- The U2AF2 /circRNA ARF1/miR-342–3p/ISL2 feedback loop regulates angiogenesis in glioma stem cells Y Jiang,Journal of Experimental & Clinical Cancer Research,2020
- Combination leflunomide and methotrexate impedes the recovery of liver fibrosis, partly through inhibition of myeloid cell admittance Mengxin Lin, et al,Molecular Medicine Reports,2019
- LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells Quan H.et al,Cell Physiol Biochem,2018
- Taxol alleviates collagen-induced arthritis in mice by inhibiting the formation of microvessels Xu J.et al,Clin Rheumatol. ,2017
- Efek Pemberian Ekstrak Biji Jarak Pagar (Jatropha curcas L) Terhadap Kadar Protein VEGF dan Gambaran Histologi Vena Sentralis Jaringan Hepar. Nabilah Aulia Hasanuddin.et al,UIN SYARIF HIDAYATULLAH JAKARTA,2016
- The Effects of Taoren-Honghua Herb Pair on Pathological Microvessel and Angiogenesis-Associated Signaling Pathway in Mice Model of CCl4-Induced Chronic Liver Disease Xi S.et al,Evidence-Based Complementary and Alternative Medicine,2016
- The effects of tao-Hong-si-Wu on hepatic necroinflammatory activity and fibrosis in a murine model of chronic liver disease Xi S. et al,J Ethnopharmacol,2016
- Biomarkers of aging, life span and spontaneous carcinogenesis in the wild type and HER-2 transgenic FVB/N female mice Panchenko AV. et al,Biogerontology,2015
- Potential Mechanism for Endothelial Progenitor Cell Therapy in Acute Myocardial Infarction: Activation of VEGF-PI3K/Akte-NOS Pathway Cheng Y et al,Ann Clin Lab Sci,2013
- Synthesized Multiple Antigenic Polypeptide Vaccine Based on B-Cell Epitopes of Human Heparanase Could Elicit a Potent Antimetastatic Effect on Human Zhang J et al,PLoS One,2013
- Erythropoietin improves the efficiency of endothelial progenitor cell therapy after myocardial infarction in mice: Effects on transplanted cell survival and autologous &hellip Cheng Y et al,Journal of Surgical Research,2012
問答及客戶評論
兩次反復(fù)凍融的具體操作方法是什么,包括冷凍和解凍的溫度以及持續(xù)時(shí)間是多少。
在組織均質(zhì)化過程中是否應(yīng)該添加蛋白酶和磷酸酶抑制劑?
用于組織勻漿所需的PBS的PH值是多少?
我們是否應(yīng)該將計(jì)算結(jié)果統(tǒng)一定為每個(gè)樣本的總蛋白質(zhì)含量?
靶點(diǎn)詳情
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最新研究進(jìn)展:VEGF-A(vascular endothelial growth factor-A)是一種重要的血管生成促進(jìn)因子,可促進(jìn)內(nèi)皮細(xì)胞增殖和血管新生。其最新研究進(jìn)展包括:在肝癌和腎癌中的高表達(dá)與預(yù)后不良相關(guān),抗VEGF-A藥物如貝伐單抗已被用于這些腫瘤的治療。此外,最近的研究還發(fā)現(xiàn)VEGF-A在血液和腎臟疾病中也起著重要的作用,如腎小球腎炎、糖尿病腎病等。
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功能:Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
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基因功能參考文獻(xiàn):
- Study using Hcar1-KO mice identified the lactate receptor Hcar1 as a key regulator of Vegf and angiogenesis in the brain and as an initial mediator of cerebral effects of physical exercise. PMID: 28534495
- Motor neurons control blood vessel patterning by an autocrine mechanism that titrates motor neuron-derived VEGF via their own expression of sFlt1. PMID: 28262664
- Targeting NLRP3 shifts the VEGF-A-induced cardiac hypertrophy from a pathologic toward a more physiologic hypertrophy. PMID: 29146733
- T3 thyroid hormone stimulates the expression and secretion of VEGF by Leydig cells PMID: 29417848
- Dexamethasone suppressed mRNA VEGF expression and VEGF production in cortical cells while in medullar cells only VEGF production was reduced. Introduction of IL-7, IL-1b or murine thymocytes increased while addition of Semaphorin 3A, SDF-1a or ACTH decreased VEGF production by cortical epithelial cells with no influence on medullar cells. PMID: 30192114
- lack of endogenous PTH may reduce VEGF expression in bone marrow mesenchymal stem cellsderived osteoblasts. PMID: 29620150
- Upregulation of podocyte VEGF decreased the number of mesangial cells via inhibition of PDGF-B-mediated signaling. PMID: 28776225
- These data provide a new pathological perspective on cerebellar astrogliosis in Niemann-Pick type C disease and suggest the importance of VEGF as a therapeutic target for this disease. PMID: 29397865
- leukocyte domiciled midkine mediates increased plasma levels of VEGFA relevant for upregulation of endothelial nitric oxide synthase 1 and 3 PMID: 29233575
- Mesenchymal stem cells secrete VEGF which in turn mediates the differentiation of endothelial progenitor cells into endothelial cells. PMID: 29138837
- This study showed that the quantity of VEGF in the glioma microenvironment seems to be crucial for the participation of microglia/macrophages on tumor progression. PMID: 28948650
- AK131850 directly competed miR-93-5p in N-OC and M-OC through sponge, thereby increasing VEGFa transcription, expression and secretion through derepressing of miR-93-5p on VEGFa. PMID: 29590659
- miR203 expression may be upregulated by IL17 stimulation, and miR203 is a positive regulator of IL17induced VEGF secretion. PMID: 29039484
- NF-kappaBmiR15abFGF/VEGFA axis contributes to the impaired angiogenic capacity of bone marrowmesenchymal stem cells in high fat dietfed mice. PMID: 28944834
- Results support the idea that excess heparin binding epidermal growth factor-like growth factor (HB-EGF) leads to a significant elevation of vascular endothelial growth factor (VEGF) and ventricular dilatation. These data suggest a potential pathophysiological mechanism that elevated HB-EGF can elicit VEGF induction and hydrocephalus. PMID: 27243144
- Over-expression of VEGF-A165b is protective against proteinuria in a mouse model with progressive depletion of all endogenous VEGF-A splice isoforms from the kidney. PMID: 28574576
- The present data suggest that Ischemic preconditioning transiently increases plasma VEGF levels by downregulating miR-762 and miR-3072-5p in CD34-positive BM cells, leading to protection against organ ischemia. PMID: 27905554
- findings are the first to demonstrate the importance of CdGAP in embryonic vascular development and VEGF-induced signaling, and highlight CdGAP as a potential therapeutic target to treat pathological angiogenesis and vascular dysfunction PMID: 27270835
- VEGF165 induces differentiation of hair follicle stem cells into endothelial cells and plays a role in in vivo angiogenesis. PMID: 28244687
- TGF-beta1/TbetaRII/Smad3 signaling pathway increased VEGF expression in in oral squamous cell carcinoma tumor-associated macrophages (TAMs). TAMs can promote the tumor angiogenesis by secreting VEGF. PMID: 29462614
- VEGF causes extensive neural stem cell (NSC) remodelling manifested in transition of the enigmatic NSC terminal arbor onto long cytoplasmic processes engaging with and spreading over even remote blood vessels, a configuration reminiscent of early postnatal "juvenile" NSCs. PMID: 27849577
- effect of dox on VEGF-A levels might at least partly explain its previously reported beneficial effects on myocardial and brain ischemia. Also, this effect on VEGF-A should be taken into account in all studies using dox-regulated vectors PMID: 29351307
- Genetic depletion experiments revealed that VEGFR2, but not VEGFR3, is indispensable for maintenance of thyroid vascular integrity. Notably, blockade of VEGF-A or VEGFR2 not only abrogated vascular remodeling but also inhibited follicular hypertrophy, which led to the reduction of thyroid weights during goitrogenesis. PMID: 28438786
- Findings suggest that VEGF gene expression can be suppressed by TNFSF15-stimulated activation of the JNK-GATA3 signaling pathway which gives rise to up-regulation of miR-29b. PMID: 27589684
- The low-molecular-weight heparin (LMWH) Tinzaparin inhibited Von Willebrand factor (VWF) fiber formation and vessel occlusion in tumor vessels by blocking thrombin-induced endothelial cells (ECs) activation and vascular endothelial growth factor-A (VEGF-A)-mediated VWF release. PMID: 27602496
- These data suggest that VEGF expressed by skeletal myofibers may directly or indirectly regulate both hippocampal blood flow and neurogenesis. PMID: 28597506
- Results show that apoE4-driven brain pathology and cognitive impairments in young apoE4 TR mice are associated with down regulation of the VEGF system and can be reversed by upregulation of the expression of VEGF in the hippocampus. These animal model findings suggest that the VEGF system is a promising target for the treatment of apoE4 carriers in Alzheimers disease. PMID: 27372644
- It was shown that peritoneal macrophages are the main suppliers of VEGF at tumor angiogenesis, as evidenced by the data obtained on model system of endothelial cells synchronized in G0/G1 phase. PMID: 29235752
- these results uncover a novel role for VEGF in controlling proper allocation of Isl1(+) cardiac progenitors to their respective descending lineages PMID: 27794491
- endothelial master transcription factor ETS1 promotes global RNAPII pause release, and that this process is governed by VEGF PMID: 28851877
- Results provide evidence that VEGF derived from Osx+ osteoblast progenitor cells is required for optimal ossification of developing mandibular bones and modulates mechanisms controlling BMP-dependent specification and expansion of the jaw mesenchyme. PMID: 26899202
- The MDA-induced VEGF increase was inhibited by autophagy-lysosomal inhibitors. Intravitreal MDA injection in mice increased laser-induced choroidal neovascularization (laser-CNV) volumes. In a mouse model fed a high-linoleic acid diet for 3 months, we found a significant increase in MDA levels, autophagic activity, and laser-CNV volumes PMID: 26923802
- deletion of AT2 receptor reduced SHP-1 activity and restored VEGF actions, leading to an increased blood flow reperfusion after ischemia in diabetes mellitus. PMID: 29074590
- ata indicate that the HIF-1alpha/VEGF-A axis is an essential aspect of tumor immunity. PMID: 29136509
- It has been concluded that stimulation of VEGF release is a key factor in the promotion of macrophage proliferation by ceramide 1-phosphate. PMID: 29080796
- 8-Br-cAMP-induced cell-secreted VEGF is biologically active and may promote angiogenesis PMID: 24493289
- results suggested that the combination of nCS (to support bone formation) with a fibrin-based VEGF/FGF9 release system (support vascular formation) is an innovative and effective strategy that significantly enhanced ectopic bone formation in vivo. PMID: 27269204
- Astrocyte-derived vascular endothelial growth factor-A (VEGF-A) is known to induce BBB dysfunction.(review) PMID: 28966265
- CRP can upregulate vascular endothelial growth factor-A (VEGF-A) expression by activating hypoxia inducible factor-1alpha (HIF-1alpha) in ADSCs. PMID: 27526687
- VEGF and IGF1 were critical factors for the spontaneous cardiac differentiation of BATDCs, and MEK/ERK signaling was involved in the role of VEGF and IGF1. PMID: 27870972
- Distal retinal ganglion cell axon transport loss and activation of p38 MAPK stress pathway following VEGF-A antagonism have been documented. PMID: 27148685
- The neuroprotection observed in ColXV KO mice may be attributed to the increased VEGF-A production following stroke in the ischemic territory. PMID: 28079884
- VEGF protein levels were also higher in the ipsilateral hemisphere of WT mice compared to Par-1 KO mice after glioma cell implantation. PMID: 26463974
- the importance of VEGF derived from tumor-infiltrating myeloid cells for initiating vascularization in gliomas PMID: 26951383
- Low VEGF expression is associated with liver fibrosis. PMID: 28118605
- We conclude that HIF-1 is not a major regulator of Vegfa expression during wound healing; rather, it serves to maintain basal levels of expression of Vegfa and its target genes in intact skin, which are required for optimal granulation tissue formation in response to wounding. PMID: 28686658
- Mechanical strain stimulates vasculogenesis of embryonic stem cells by the intracellular messengers ROS, NO and calcium as well as by upregulation of angiogenesis guidance molecules and the angiogenic growth factors VEGF, FGF-2 and PDGF-BB. PMID: 27725190
- This study identifies YAP/TAZ as central mediators of VEGF signaling PMID: 28867486
- Ectopic midline vascularisation in endothelial Nrp1 and Vegfa(188/188) mutants caused additional axonal exclusion zones within the chiasm. PMID: 28676569
- VEGF inhibition decreases local CFH and other complement regulators in the eye and kidney through reduced VEGFR2/PKC-alpha/CREB signaling. PMID: 27918307
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亞細(xì)胞定位:[Isoform VEGF-1]: Secreted.; [Isoform VEGF-2]: Secreted.; [Isoform VEGF-3]: Cell membrane; Peripheral membrane protein. Note=Remains cell-surface associated unless released by heparin.
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蛋白家族:PDGF/VEGF growth factor family
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組織特異性:In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in ki
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