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  4. Mouse Follistatin,FS ELISA Kit

Mouse Follistatin,FS ELISA Kit

  • 中文名稱：
    小鼠卵泡抑素(FS)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08508m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse FST ELISA Kit was designed for the quantitative measurement of Mouse FST protein in serum, plasma, tissue homogenates, cell lysates. It is a Competitive ELISA kit, its detection range is 0.05 ng/mL-10 ng/mL and the sensitivity is 0.025 ng/mL .

  • 別名：
    FstFollistatin ELISA Kit; FS ELISA Kit; Activin-binding protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.05 ng/mL-10 ng/mL
  • 靈敏度：
    0.025 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse follistatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-100  
    1:2 Average % 88  
    Range % 83-91  
    1:4 Average % 90  
    Range % 86-94  
    1:8 Average % 101  
    Range % 97-105  
  • 回收率：
    The recovery of mouse follistatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 91 87-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    10 0.221 0.225 0.223    
    2.5 0.342 0.351 0.347    
    0.8 0.533 0.546 0.540    
    0.2 0.798 0.778 0.788    
    0.05 1.244 1.223 1.234    
    0 2.317 2.163 2.240    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

問答及客戶評論

■ 常見問題解答
Q:

我想測定小鼠EDTA血漿中的卵泡抑制素水平（CSB-E08508m）。請問如何稀釋血漿？

A:
我們通常直接檢測樣本血清/血漿而不進(jìn)行稀釋，考慮到樣品的差異（樣品采集的生理階段和儲存的差異等），最好先探索梯度條件：未稀釋，10倍，100倍稀釋，使用1×PBS進(jìn)行預(yù)實(shí)驗(yàn)。

靶點(diǎn)詳情

  • 功能：
    Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
  • 基因功能參考文獻(xiàn)：
    1. These data suggest that activin-follistatin regulation may play a role during the development of Experimental autoimmune epididymo-orchitis. PMID: 28205525
    2. Uterine Fst-cKO mice demonstrate severe fertility defects and deliver only 2% of the number of pups delivered by control females. In Fst-cKO mice, the uterine luminal epithelium does not respond properly to estrogen and progesterone signals and remains unreceptive to embryo attachment by continuing to proliferate and failing to differentiate. PMID: 28559342
    3. Data suggest that the level of circulating follistatin is involved in testicular damage due to autoimmune orchitis; these studies were conducted using gene transfer to increase circulating levels of follistatin in experimental model of autoimmune orchitis. PMID: 28667125
    4. Surface plasmon resonance analysis showed no significant association between FS288 and AMHC , suggesting that FS288 indirectly regulates AMH signaling. Activin A, a direct target of FS288, did not itself induce reporter activity in P19 cells, but did prevent the FS288-induced increase in AMH signaling. Hence, local concentrations of FS288 and Activin A may influence the response of some cell types to AMH. PMID: 28500669
    5. Hypergravity affects follistatin levels in muscle through the vestibular system in mice. Follistatin may play some roles in the interactions between muscle and bone metabolism in response to gravity change. PMID: 28471505
    6. Fst promotes brown adipocyte characteristics in both white adipose tissue and brown adipose tissue depots in vivo through distinct mechanisms. PMID: 28324027
    7. Results showed activin-C and follistatin are differentially expressed during prostate development and suggested that the antagonistic property of follistatin is secondary to the action of activin-C. Study provides evidence to support a role of activin-C in prostate development and provides new insights in the spatiotemporal localization of activins and their antagonists during mouse prostate development. PMID: 28363632
    8. Oviducts of tghFST315 mice failed to coil, the myometrium was disorganised, endometrial gland number was reduced and oviducts and uteri contained abundant leukocytes. These studies show that follistatin is crucial to postnatal oviductal-uterine development and function. PMID: 24630125
    9. OVOL1-regulated Fst and SFRP1 affect hair inductive potency of neonatal dermal cells. PMID: 25827793
    10. This study assesses the regulation of activin A and follistatin in a murine myocardial ischaemia-reperfusion model. PMID: 25052838
    11. Results implicate a role of follistatin (Fst) in the induction of brown adipocyte character and regulation of energy metabolism. PMID: 24443561
    12. follistatin induces hair wave propagation and its level decreases in aging mice. PMID: 24618599
    13. Follistatin aids in maintaining proper somite size, and consequently sclerotome progenitor numbers, by preventing paraxial mesoderm from adopting an intermediate/lateral plate mesodermal fate in the Noggin-deficient state. PMID: 24514266
    14. Report role of activin A-myostatin-follistatin system on aging bone and muscle progenitor cells. PMID: 23178301
    15. Although it is a promising therapeutic tool in chronic or acute inflammatory conditions not caused by virulent pathogens, FS does not seem to increase the resistance to bacterial infections. PMID: 23143061
    16. Data from transgenic mice suggest that overexpression of follistatin negatively influences bone metabolism decreasing all measured biomechanical strength parameters in developing bone. PMID: 22311089
    17. These data indicate that the rapid increase in circulating activin A during LPS-induced inflammation is regulated at the posttranscriptional level. PMID: 22855279
    18. regulation of Smad3- and mTOR-dependent events by follistatin occurred independently of overexpression or knockout of myostatin, a key repressor of muscle development that can regulate Smad3 and mTOR signaling and that is itself inhibited by follistatin PMID: 22711699
    19. We conclude that testosterone stimulation of satellite cell proliferation and myogenic differentiation are associated with up regulation of Fst and inhibition of TGF-beta-signaling. PMID: 22138414
    20. follistatin stimulated myoblasts to express MyoD, Myf5, and myogenin, which are myogenic transcription factors that promote myogenic differentiation PMID: 21689628
    21. These results demonstrate that body composition and glucose homeostasis are differentially regulated by FST and FSTL3 and that their combined loss is associated with increased fat mass and insulin resistance despite elevated insulin production. PMID: 21546932
    22. gradual increase in muscle regeneration, production stimulated in the presence of necrosis PMID: 21416223
    23. the transition between stem cells and committed progenitors is neither sharp nor irreversible and GDF11, ACTbetaB and FST are crucial components of a circuit that controls both total cell number and the ratio of neuronal versus glial cells in this system PMID: 21852401
    24. Results show that FST is critical for normal regulation of germ cell nest breakdown and that loss of the FST303 and/or FST315 isoforms leads to excess primordial follicles with accelerated demise, resulting in premature cessation of ovarian function. PMID: 21106872
    25. Changes in hepatically metabolised follistatin during and after exercise are reported. PMID: 21068158
    26. Data show that follistatin mutant mice exhibit haploinsufficiency, with muscles of reduced size, a shift toward more oxidative fiber types, impairment of muscle remodeling in response to injury, and reduction in tetanic force production. PMID: 20810712
    27. link between mTOR and miRNA biogenesis; an mTOR-miR-1-HDAC4-follistatin pathway that regulates myocyte fusion during myoblast differentiation in vitro and skeletal muscle regeneration in vivo. PMID: 20566686
    28. The FST288-only isoform is sufficient for development. Loss of FST303 and FST315 isoforms results in fertility defects that resemble activin hyperactivity and premature ovarian failure. PMID: 20032047
    29. we studied the expression of follistatin in rat and mouse liver tumors as a possible mechanism to overcome activin growth control, 40% of the tumors displayed increased levels of follistatin mRNA when compared to tumor-surrounding liver tissue. PMID: 12203361
    30. up-regulation of the follistatin mRNA level in the small myotubes of the regenerating soleus as well as the down-regulation of the myostatin transcript level after denervation contribute to the differentiation process in denervated regenerating muscle. PMID: 12761853
    31. Data suggest that Follistatin antagonises Myostatin by direct protein interaction, which prevents Myostatin from executing its inhibitory effect on muscle development. PMID: 15136138
    32. germ cells in the ovarian cortex are almost completely lost in both Wnt4 and Fst null gonads before birth PMID: 15162500
    33. review with emphasis on authors' works on paracrine and autocrine regulation in activities of Fst and Fstl3, regulation of gonadal development, and structural elements necessary for biological activities PMID: 15451564
    34. experiments provide evidence that GnRH stimulates follistatin through multiple signaling pathways PMID: 17482756
    35. repression of Sp1 transcriptional activity by PPARgamma is the underlying mechanism responsible for PPARgamma-mediated regulation of follistatin expression PMID: 18768463
    36. Antral hyperplasia in Gas-/- mice involves amplification of mucous cell lineages due to follistatin, suggesting its role in the development of antral gastric tumors. PMID: 18804092
    37. This review summarises the activin signal transduction pathways and the interactions between activins and other TGFbeta signalling systems during hair follicle formation, hair growth cycling, skin function and wound healing.[review] PMID: 18922734
    38. Keratinocyte-derived follistatin regulates epidermal homeostasis and wound repair. PMID: 19079322
    39. FoxL2 and Smad3 coordinately regulate follistatin gene transcription.( PMID: 19106105
    40. Satellite cell proliferation and both Mstn and Act inhibition are involved in the FS-induced muscle hypertrophy. PMID: 19435857
    41. This study examined the expression of myostatin and follistatin in Mdx mice and B10 mice; a temporary and coincident expression of follistatin and myostatin in both mouse strains, but this expression was significantly higher in Mdx mice. PMID: 19715499

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  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：

    KEGG: mmu:14313

    STRING: 10090.ENSMUSP00000022287

    UniGene: Mm.4913