Mouse Angiotensin converting enzyme,ACE ELISA Kit
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中文名稱:小鼠血管緊張素轉(zhuǎn)化酶(ACE)酶聯(lián)免疫試劑盒
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貨號:CSB-E04492m
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:血管緊張素轉(zhuǎn)化酶(ACE)是一種重要的生物酶,它可以幫助人體控制血壓。ACE有兩個主要的功能:一是將血管緊張素I轉(zhuǎn)化成血管緊張素II,二是抑制血小板聚集。它可以通過將血管緊張素I轉(zhuǎn)化為血管緊張素II來改變血壓水平。 ACE也可以幫助人體抵御感染,因?yàn)樗軌虼龠M(jìn)免疫應(yīng)答的形成。華美生物所提供的Mouse Angiotensin converting enzyme,ACE ELISA Kit屬于ELISA檢測試劑盒,采用雙抗夾心法定量檢測鼠血清、血漿、組織勻漿樣本中的ACE,其靈敏度為0.39 ng/mL,檢測范圍為1.56 ng/mL-100 ng/mL。
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別名:Ace ELISA kit; Dcp1Angiotensin-converting enzyme ELISA kit; ACE ELISA kit; EC 3.2.1.- ELISA kit; EC 3.4.15.1 ELISA kit; Dipeptidyl carboxypeptidase I ELISA kit; Kininase II ELISA kit; CD antigen CD143) [Cleaved into: Angiotensin-converting enzyme ELISA kit; soluble form] ELISA kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 95 Range % 84-101 1:10 Average % 96 Range % 86-103 1:20 Average % 96 Range % 92-100 1:40 Average % 95 Range % 87-101 -
回收率:
The recovery of mouse ACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 92-103 EDTA plasma (n=4) 92 85-98 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.153 2.141 2.147 1.971 50 1.542 1.596 1.569 1.393 25 1.117 1.104 1.111 0.935 12.5 0.703 0.688 0.696 0.520 6.25 0.489 0.474 0.482 0.306 3.12 0.322 0.318 0.320 0.144 1.56 0.259 0.266 0.263 0.087 0 0.172 0.180 0.176 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Potential role of angiotensin converting enzyme/neprilysin pathway and protective effects of omapatrilat for paracetamol?induced acute liver injury ZB Aksakalli?Magden,Experimental and Therapeutic Medicine,2022
- Possible contribution of the neprilysin/ACE pathway to sepsis in mice RA Ugan,Life Sciences,2020
產(chǎn)品評價
樣品類型:血清
樣品信息:小鼠
稀釋比:其他 1:20
產(chǎn)品評價: 我用CSB-E04492m分別檢測了造模及治療前后小鼠血清中ACE的水平含量,發(fā)現(xiàn)治療之后小鼠血清中ACE水平下降了。第一次接觸ELISA,有很多操作細(xì)節(jié)不清楚,聯(lián)系了他們售后,非常耐心地一一作了解答了,幫忙處理處理數(shù)據(jù),方便好多。
By 申老師
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:血管緊張素轉(zhuǎn)化酶(Angiotensin Converting Enzyme,ACE)是一種重要的酶類分子,具有調(diào)節(jié)血管收縮、水鹽代謝、免疫反應(yīng)等多種生理作用。近年來,對ACE的研究逐漸深入,以下是最新的研究進(jìn)展:ACE在疾病中的作用機(jī)制得到了深入研究。例如,ACE與高血壓、冠心病、肺動脈高壓等疾病的關(guān)聯(lián)性研究已經(jīng)得到了廣泛關(guān)注。研究人員發(fā)現(xiàn),ACE可以調(diào)節(jié)血管緊張素的生成,進(jìn)而影響血管的收縮和擴(kuò)張,從而對心血管系統(tǒng)的功能產(chǎn)生影響。ACE的代謝和信號轉(zhuǎn)導(dǎo)途徑研究取得了新進(jìn)展。研究人員已經(jīng)發(fā)現(xiàn)多種ACE受體,并且探索了它們與ACE的結(jié)合機(jī)制。同時,ACE代謝途徑中的關(guān)鍵酶類的作用機(jī)制也得到了更深入的研究。ACE在藥物研發(fā)中的應(yīng)用也取得了一定進(jìn)展。例如,ACE抑制劑已經(jīng)成為治療高血壓和心血管疾病的一類重要藥物。此外,研究人員還通過對ACE的結(jié)構(gòu)和功能的研究,探索了一些新型ACE抑制劑的設(shè)計和合成方法。ACE與免疫系統(tǒng)的關(guān)系也引起了研究人員的關(guān)注。研究發(fā)現(xiàn),ACE不僅能夠調(diào)節(jié)血管緊張素的生成,還能夠調(diào)節(jié)多種細(xì)胞因子的生成,從而影響免疫反應(yīng)的進(jìn)行。
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功能:Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
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基因功能參考文獻(xiàn):
- ACE expression/phosphorylation in the bone-marrow niche interface negatively regulates G-CSF-induced signaling and hematopoietic progenitor cell mobilization. PMID: 29549541
- first evidence indicating the causation between ACE DD or B2R+9bp genotype and the increased risk for diabetic nephropathy, broadening our horizon about the role of genetic modulators in this disease. PMID: 28390948
- a small increase in angiotensin-converting enzyme activity in diabetic animals leads to greater impairment of autonomic function, as demonstrated by increased sympathetic modulation and reduced cardiac vagal modulation along with increased renal expression of Ang II. PMID: 30088535
- Resveratrol upregulated ACE2 and inhibited abdominal aortic aneurysm growth in a mouse model. PMID: 28935757
- In mice with renal injury induced by L-NAME pretreatment, renal tubular epithelial ACE is essential for renal angiotensin II accumulation and salt-sensitive hypertension PMID: 27988209
- Studied ACE role in peptide processing and for MHC class II antigen presentation; found ACE level effects efficiency of antigen presentation, and overexpression or inhibition of ACE alters the CD4(+) T-cell and antibody response. PMID: 28394320
- ACE enhances the oxidative response and bactericidal activity of neutrophils. PMID: 28515091
- this study shows that angiotensin-converting enzyme inhibitor captopril rescues mice from endotoxin-induced lethal hepatitis PMID: 27879415
- Smooth muscle cell-derived ACE contributes to atherosclerosis, independent of circulating ACE activity and blood pressure. PMID: 27055902
- Intestinal ACE shedding is increased by DSS-induced intestinal inflammation and parallels local corticosterone production. ACE product angiotensin II stimulates corticosterone formation in healthy intestine. PMID: 26401072
- Renin angiostensin system contributes to 20-HETE-mediated microvascular remodeling in hypertension and that 20-HETE-driven microvascular remodeling independent of blood pressure elevation does not fully rely on ACE activity in the vascular endothelium. PMID: 25924878
- angiotensin-converting enzyme has an essential role in hypertension induced by nitric oxide synthesis inhibition PMID: 25012170
- Suggest that the ACE2-ACE imbalance plays an important role in the pathogenesis of severe acute pancreatitis and that pancreatic ACE2 is an important factor in determining the severity of SAP. PMID: 24414175
- Show that tissue-specific targets are critical for the effects of miR-143/145 on smooth muscle differentiation and that angiotensin converting enzyme is one such target. PMID: 25273883
- Tissue-specific expression of transgenic secreted ACE in vasculature can restore normal kidney functions, but not blood pressure, of Ace-/- mice. PMID: 24475296
- ACE-null mice produce large numbers of myeloid-derived suppressor cells during chronic inflammation. PMID: 24614194
- Myelomonocytic expression of catalytically active ACE, prevents cognitive decline in a murine model of Alzheimer disease. PMID: 24487585
- ACE mediates angiotensin I-induced atherosclerosis, and ACE expression in leukocytes modestly contributes to atherosclerotic development in hypercholesterolemic mice. PMID: 23846498
- TEX101 is a unique specific substrate for ACE that is essential for the production of fertile mouse spermatozoa PMID: 23633567
- renal ACE activity is required to increase local Ang II, to stimulate sodium transport in loop of Henle and the distal nephron, and to induce hypertension PMID: 23619363
- deregulation of ACE and ACE2 plays an important role in tourniquet-induced renal injury PMID: 22580272
- When Ang I and CMP48/80 were co-administered, AT(1B) receptor expression was increased, MCP-4 was found surrounding the vessel wall PMID: 21622682
- shapes the MHC class I peptide repertoire for antigen presentation to T-cells PMID: 21964607
- Mature adipocytes modulate the expression profile of macrophages by releasing lipid mediators that increase ACE expression. PMID: 21116821
- results demonstrate a previous unrecognized significant role for ACE in myelopoiesis and imply new perspectives for manipulating myeloid cell expansion and maturation. PMID: 21148418
- Leptin regulates ACE activity in mice PMID: 20614101
- Angiotensin-converting enzyme overexpression in mouse myelomonocytic cells augments resistance to Listeria and methicillin-resistant Staphylococcus aureus PMID: 20937811
- A modest genetic increase in ACE impairs myocardial tolerance to ischemia. ACE level plays a critical role in cardiac ischemia, through both kinin and angiotensin mediated mechanisms. PMID: 20667972
- data provide direct evidence that small genetic disturbances in ACE levels per se have an influence on haemodynamic, cardiac mass and autonomic nervous system responses in mice under pathological perturbation PMID: 19930431
- angiotensin-converting enzyme has a role in improving autonomic dysregulation in ob/ob mice PMID: 20012594
- data suggest that the two catalytic domains of mACE do not function independently but that the signal transduction is influenced by negative cooperativity of the two catalytic domains PMID: 20030584
- There is a switch from ACE-dependent to serine protease-dependent ANG II formation in the type II diabetic kidney. PMID: 19846569
- data suggest that ACE is essential to ANG II formation in the vascular space PMID: 12003835
- urine-concentrating defect in mice with absence of tissue ACE is associated with downregulation of key urea, salt, and water transport proteins PMID: 12167603
- Chymase may play a role in heart remodeling by increasing Ang II formation and activating MMP-9, and the regulation of collagen I gene expression. PMID: 12359984
- gender differences in expression of ACE in the heart PMID: 12384478
- results demonstrated that the rodent germinal angiotensin converting enzyme is released from the testicular sperm membrane when sperm enter the epididymis PMID: 12444051
- There is no any association between angiotensin converting enzyme polymorphism and Alzheimer's disease. PMID: 12459519
- Substantial evidence is provided for a major role for angiotensin converting enzyme in a murine allergic contact dermatitis model of neurogenic infolammation. PMID: 12646655
- Expression of ACE, either in renal proximal tubules or in vasculature, is sufficient for maintaining normal kidney functions, but for maintaining blood pressure, ACE must be expressed in vascular endothelial cells. PMID: 12777443
- the presence of the C-terminal ACE catalytic domain is sufficient to maintain a functional renin-angiotensin system PMID: 14757757
- ACE is the predominant pathway of angiotensin II formation in blood and tissues of mice and plays a major role in bradykinin (1-9) metabolism in blood and, to a lesser extent, in kidney and heart. PMID: 14769811
- t-ACE contributes to arterial structure/remodeling & hyperplastic carotid inward remodeling from blood flow cessation. It is not needed for outward hypertrophic & subsequent inward hypotrophic remodeling of the uterine artery during/after pregnancy. PMID: 15031129
- Aldosterone upregulated macrophage ACE mRNA expression and activity. The selective effect of aldosterone on tissue ACE, versus circulating ACE, further demonstrates differences in the regulation pathways of tissue and circulating ACE. PMID: 15123520
- ACE Tyr phosphorylation, probably in the endoplasmic reticulum, enhances the rate of its cleavage secretion at the plasma membrane using a regulatory pathway that may involve p38 MAP kinase PMID: 15252021
- Mice with cardiac-restricted angiotensin-converting enzyme have atrial enlargement, cardiac arrhythmia, and sudden death. PMID: 15331425
- Ptn has the potential to critically regulate the downstream activities of angiotensin II, through the regulation of its synthesis by ACE and its receptor mediated functions through regulation of both the AT1 and AT2 receptors PMID: 15485659
- renal tissue angiotensin converting enzyme is an important contributor to tubuloglomerular feedback PMID: 15494545
- ACE is associated with body weight and peri-epididymal fat accumulation PMID: 15522949
- ACE signaling may underlie the increase in COX-2 and prostacyclin levels in patients treated with ACE inhibitors. PMID: 15569856
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亞細(xì)胞定位:[Angiotensin-converting enzyme, soluble form]: Secreted.; Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Detected in both cell membrane and cytoplasm in neurons.
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蛋白家族:Peptidase M2 family
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組織特異性:Testis-specific isoform is expressed in spermatocytes, adult testis.
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