Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit
產品詳情
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產品描述:
BGLAP蛋白,全名為骨γ-羧基谷氨酸蛋白(Bone gamma-carboxyglutamic acid protein),是一種在骨組織中表達的蛋白質。它是一種含有大量谷氨酸殘基和γ-羧基谷氨酸殘基的糖蛋白,具有較高的親和力和親和度,能夠結合到骨礦質上。
BGLAP蛋白在骨代謝中發(fā)揮著重要的作用。它是一種調節(jié)鈣離子轉運和骨重建的關鍵因子。BGLAP蛋白在骨細胞分化和成熟過程中的表達量與骨密度的增加有關。它能夠促進骨細胞的分化和骨重建,從而提高骨密度和強度。
此外,BGLAP蛋白也被認為是骨代謝中的早期標志物。它可以作為骨轉換的指標,幫助評估骨健康狀況和預測骨質疏松癥的發(fā)生風險。BGLAP蛋白還與一些骨相關疾病,如骨質疏松癥、骨轉移性腫瘤等有關。
華美生物所提供的Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit屬于ELISA檢測試劑盒,采用雙抗夾心法定量檢測人血清、血漿、組織勻漿樣本中的BGLAP,其靈敏度為7.8 pg/ml,檢測范圍為31.25 pg/ml-2000 pg/ml。
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別名:
BGLAP ELISA Kit; BGP ELISA Kit; Bone gamma carboxyglutamate (gla) protein ELISA Kit; Bone gamma carboxyglutamate gla protein osteocalcin ELISA Kit; Bone gamma carboxyglutamate protein ELISA Kit; Bone Gla protein ELISA Kit; Gamma carboxyglutamic acid containing protein ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit; OC ELISA Kit; OCN ELISA Kit; OSTCN_HUMAN ELISA Kit; Osteocalcin ELISA Kit; OTTHUMP00000016586 ELISA Kit; PMF1 ELISA Kit
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縮寫:
BGLAP
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Uniprot No.:
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種屬:
Homo sapiens (Human)
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樣本類型:
serum, plasma, tissue homogenates
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檢測范圍:
31.25 pg/ml-2000 pg/ml
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靈敏度:
7.8 pg/mL
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反應時間:
1-5h
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樣本體積:
50-100ul
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檢測波長:
450 nm
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研究領域:
Metabolism
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測定原理:
quantitative
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測定方法:
Sandwich
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精密度:
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線性度:
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回收率:
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標準曲線:
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本試劑盒所含材料:
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human BGLAP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled BGLAP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料:
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理:
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貨期:
3-5 working days
引用文獻
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Effectiveness of MIST with hydroxyapatite and β-tricalcium phosphate in alveolar bone density and osteocalcin level improvement during treatment of infrabony pockets
AA Mubarokah,Majalah Kedokteran Gigi Indonesia,2022
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Does Serum Osteocalcin Level Affect Carotid Atherosclerosis in Post-Menopausal Diabetic Females? A Case-Control Study
M Naguib,International journal of general medicine,2022
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Is There a Balance in Oxidative-Antioxidant Status in Blood Serum of Patients with Advanced Endometriosis?
I Kokot,Antioxidants,2021
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PGC-1α attenuates the oxidative stress-induced impaired osteogenesis and angiogenesis regulation effects of mesenchymal stem cells in the presence of diabetic serum
Zongxin Shi,Biochemistry and Biophysics Reports,2021
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Pulsed electromagnetic fields ameliorate skeletal deterioration in bone mass, microarchitecture and strength by enhancing canonical Wnt signaling-mediated bone formation in rats with spinal cord injury
X Shao,Journal of Neurotrauma,2020
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Irisin ameliorates bone loss in ovariectomized mice
Y Luo,Climacteric,2020
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Curative Effects of Zoledronic Acid Combined with Calcitonin on the Treatment of Bone Metastasis and Osteoporosis in Lung Cancer
Chang-yan Xu.et al,International Journal of Pharmacology,/
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Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells
Manaka S.et al,FEBS Lett.,2015
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Fetuin a promotes lipotoxicity in ß cells through the TLR4 signaling pathway and the role of pioglitazone in anti-lipotoxicity
Shen X. et al,Mol Cell Endocrinol,2015
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Expression of porcine Mx1 with FMDV IRES enhances the antiviral activity against foot-and-mouth disease virus in PK-15 cells
Yuan B. et al,Arch Virol,2015
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Serum osteopontin levels in relation to bone mineral density and bone turnover markers in postmenopausal women
Wei QS. et al,Scand J Clin Lab Invest,2015
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Effects of Polygonatum sibiricum polysaccharide on the osteogenic differentiation of bone mesenchymal stem cells in mice
Zong S. et al,Int J Clin Exp Pathol,2015
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EFFECTS OF LIGHT SMOKING ON SALIVARY LEVELS OF ALKALINE PHOSPHATASE AND OSTEOCALCIN IN CHRONIC PERIODONTITIS PATIENTS
Lubaba R Abdul Ameer. et al,journal of baghdad college of dentistry,2015
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Association between osteocalcin and metabolic syndrome in postmenopausal women
Lee SW et al,Arch Gynecol Obstet,2015
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