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  4. Human Cortisol ELISA Kit

Human Cortisol ELISA Kit

  • 中文名稱：
    人皮質(zhì)醇(Cortisol)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E05111h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 縮寫：
    COR
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, saliva.
  • 檢測范圍：
    3.12 ng/ml-200 ng/ml.
  • 靈敏度：
    1.56 ng/ml.
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
     
  • 線性度：
     
  • 回收率：
     
  • 標準曲線：
     
  • 本試劑盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
      • Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
      • Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
      • Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
      • HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
      • Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
      • TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
      • Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：生物流體 唾液

樣品信息：人

稀釋比：其他 1：4

產(chǎn)品評價： 我用csb-E05111h檢測人類唾液皮質(zhì)醇，od值為1.5285，濃度為23.09，試劑盒整體標準曲線較好，比較穩(wěn)定。

By 王老師

問答及客戶評論

■ 常見問題解答
Q:

I would like to know if corticosterone also affects to OD value.

A:
Thanks for your inquiry.
This kit is specific to Cortisol.It has no correlation with corticosterone and the corticosterone does not affect OD value.
Q:

I hope you would be able to advice me which kit would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on cortisol. Sample size is 60 and running in
duplicates.

A:
Thanks for your inquiry.
We have CSB-E05111h available. It is based on competitive method. It is suitable to test human salivary. Pls folow actual test result.
One 96T kit can test 43 samples if you run in duplicates.
■ 客戶評論
  • Sample type: Plasma
    Sample species: Human
    Review: Cortisol levels in Experiment 2. Food, n = 21; Fasting, n = 24. b Cortisol levels in Experiment 4. Food, n = 22; Fasting, n = 23. Blue dots and red dots represent individuals in the food group and fasting group, respectively.
    PMID: 30301955
  • Sample type: Plasma
    Sample species: Human
    Review: Data are expressed as means SEM, except plasma cortisol and epinephrine concentration, which are descripted as median (inter-quartile range, IQR). FEV1, forced expiratory volume in one second; FVC, forced vital capacity.
    PMID: 30937140
  • Sample type: Saliva
    Sample species: Human
    Review: Cortisol responses to the Trier Social Stress Test by bullying group. Cortisol was measured in nanograms per milliliter (ng/ml), and was log-transformed in this figure. Figure shows means for cortisol levels, for physical bullied, verbal bullied, relational bullied, the total bullied and nonbullied children.
    PMID: 29196916
  • Sample type: Serum
    Sample species: Human
    Review: FPG, fasting plasma glucose; PPG, postprandial plasma glucose; BMI, body mass index; p < 0.05 denoted as a statistically significant (in bold). *Student t test, *Type 1 diabetes vs controls p < 0.05, #Type 1 vs Type 2 diabetes, p < 0.05, **Type 2 diabetes vs controls p < 0.05.
    PMID: 30184546

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