- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human C4 antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated C4 antibody (100 x concentrate) (120 μl/bottle) ---Bind to the C4 in the samples or standards and react with the substrate to make the solution chromogenic.
- One HRP-conjugate Diluent(20 ml/bottle) ---Dilute the HRP-conjugated C4 antibody
- Two vials Sample Diluent (20 ml/bottle) ---Dilute the sample solution.
- One vial Wash Buffer (25x concentrate) (20ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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