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Fish trypsin ELISA Kit

  • 中文名稱：
    魚胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018811FI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：

    This Fish PRSS1 ELISA Kit was designed for the quantitative measurement of Fish PRSS1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.156 ng/mL.

  • 別名：
    Trypsin-1 ELISA kit; EC 3.4.21.4 ELISA kit; Trypsin I ELISA kit
  • 縮寫：
    PRSS1
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 101
    Range % 95-105
    1:40 Average % 91
    Range % 84-95
    1:80 Average % 106
    Range % 100-111
    1:160 Average % 104
    Range % 97-108
  • 回收率：
    The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 87-100
    EDTA plasma (n=4) 102 96-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    20 0.231 0.227 0.229
    10 0.342 0.337 0.340
    5 0.497 0.482 0.490
    2.5 0.663 0.645 0.654
    1.25 0.932 0.918 0.925
    0.625 1.300 1.246 1.273
    0.312 1.774 1.724 1.749
    0 2.434 2.389 2.412
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 亞細胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 數據庫鏈接：

    KEGG: sasa:100137021

    UniGene: Ssa.38456